Study Cytotoxic Effect Of Cisplatin Derivatives Or Drugs On Human Cancerous Cell Lines

Abas O. Hadi
Department of optics, College of Health & Medical Technology, Sawa University, Samawah,
Iraq.

Abstract

Cell culture studies are very important and desirable, as they provide a system to study mechanism,
direct access ,and evaluation of tissues. The technique of tissue culture is a valuable tool to study
problems of clinical relevance, especially those related to diseases, screening, and studies of cell
toxicity mechanisms. In the present study, heterocyclic aromatic cisplatin derivatives were used.
Four cell lines viz. HepG2, ZR-75, HL-60, and Daudi were cultures in Dulbecco’s Modified Eagle’s
medium (DMEM) medium. The cells were harvested after 24hr incubation. Various concentrations
(10-100µl) of these eight cisplatin derivatives were used to determine their effect on cell
proliferation. MTT- cell cytotoxicity assay was used to determine the effect of these cisplatin
derivatives on various cell lines, the cytotoxic effect of eight cisplatin derivatives viz. 1100.001,
1100.002, 1100.003, 1100.004, 1100.006, 1100.008, 1100.009 and 1100.013 on cancerous cell lines
by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Those selected cell
lines are ZR-75, HL-60, Daudi, and HepG2. Various concentration of each compound was
evaluated for this study.
Key words: cell lines, MTT assay, DMEM

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