Molecular Detection and Genotypic Study of Eggerthella -Like Uncultured Bacterium by 16SrRNA Gene that Isolated from Bacterial Vaginosis Women with and without-Miscarriage in AL-Hillah City.
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January 25, 2024Alaa K . Hameed¹ , Ilham A . Bunyan² and Asmaa K. Gatea²
¹ Department of Radiology, College of Health and Medical Techniques, Sawa University ,Almuthana, Iraq.
²Department of Microbiology, College of Medicine, University of Babylon , AL-Hillah, Iraq
Abstract
The present study was conducted to determine Eggerthella spp by culture independent method from both bacterial vaginosis(BV) women without and without miscarriage . Another aim was to sequence the 16SrRNA gene for phylogenetic study of the local isolates of Eggerthella spp in comparison to world Eggerthella spp isolates in NCBI Gen bank and lastly deposition of the current isolates in Gen bank. Hundred and fifty (150) high vaginal swabs from BV women with and without miscarriage were collected from the Hospital for Maternity and children and private clinics of Babylon city , where Seventy five samples(75) were taken from married BV women without miscarriage and Seventy five samples (75) from BV women with miscarriage . The patient’s age (15– 45) years. The samples was taken by disposable swabs, 16SrRNA gene detection by polymerase chain reaction technique. Results revealed that from a total 150 swabs, 31(41.33%) and 18(24.00%) of Eggerthella spp obtained by PCR from BV women with and without -miscarriage respectively. Phylogenetic study of 16SrRNA gene has shown that local isolates Eggerthella spp (NO.1 and NO.2 ) have shared a higher homology with other isolates of Eggerthella spp available in the GenBank. Sequence similarity was (98.51% and 98.31% ) for isolates (No.1 and No.2) respectively.
