Evaluation level hormones and GSTM1, GSTT1 polymorphism in infertility Among Iraqi men’s

Saheb J . Al Fleafil¹ , Abdul Hussein M.Al Faisal ² , Rasha A. Mahood²
¹Department of Medical Laboratories,College of Health and Medical Techniques, Sawa University ,Almuthana, Iraq.
2 Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Iraq.

Abstract

Several studies refer to higher levels of oxidative stress are associated with infertility. The present study included 100
Iraqi men who were diagnosed as prostate cancer patients. The age of the patients ranged from 20 to 40 years beside
50 who wereApparently healthymen. Participated in this study. Certain semen parameters were investigated according
to World Health Organization (WHO) guidelines. Follicle –stimulating hormone (FSH), Luteinizing hormone (LH),
Prolactin hormone and testosterone levels were also determined by enzyme –linked immunosorbent assay (ELISA).
The purpose of this study to know the frequency of GSTM1 and GSTT1 genes in infertility patients and asset the
relationship between the genes and some level hormones in infertility patients. Genotype (GSTM1 and GSTT1) genes
were determined by polymerase chain reaction (PCR). The results showed that 36 samples of patients
(oligozoospermia) have deletion in one genes or both (Null genotype), whereas 14 samples only were normal. where,
results showed that 34 samples of patients (asthenozoospermia) have deletion in one genes or both (Null genotype),
whereas 16 samples only were normal. For control group, 50 samples were collected from apparently healthy men
from different areas in dewania. Only eight samples showed genetic deletion while 42 samples were normal genotype.
Hormones level. There is no significant different between oligozoospermia and asthenozoospermia cases & control
group in regard to FSH, LH, Prolactin, and testosterone level.
Keywords: Infertility, GSTM1, GSTT1, polymorphism. Hormones.

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